At the height of his killing spree in the 1970s, the psychopath was executed in 1989, would feign a broken arm or leg and ask young women for help before murdering them.
But at the time of his convictions DNA typing was not widely used, but now the advances in the technology have enabled investigators to link serial killers like Bundy, to unsolved crimes.
His blood, recovered from an evidence lab in Columbia County, Florida, will be entered into the FBI's Combined DNA Index System (CODIS) on Friday - something investigators are calling a major milestone.
They now hope it will help them complete his awful profile.
A reopened 1961 cold case involving a missing 8-year-old girl in Tacoma, Washington, was the impetus for the completed DNA profile.
The girl lived along a newspaper route that the then-teenager Bundy worked, according to published accounts.
Florida Department of Law Enforcement spokesman Keith Kameg said: "A profile will assist with whatever evidence you have. It doesn't mean someone did it, but it does mean they were there.
"The technology in the past 25 years went from archaic to phenomenal,"
He added that his department contacted law enforcement agencies across the state seeking any evidence that could be used to build a profile on Bundy.